|
KMID : 0545120230330101361
|
|
Journal of Microbiology and Biotechnology
2023 Volume.33 No. 10 p.1361 ~ p.1369
|
|
|
Crystal Structures of 6-Phosphogluconate Dehydrogenase from Corynebacterium glutamicum
|
|
Yu Hyeon-Jeong
Hong Ji-Yeon
Seok Ji-Hye
Seu Young-Bae
Kim Il-Kwon
Kim Kyung-Jin
|
|
|
Abstract
|
|
|
|
Corynebacterium glutamicum (C. glutamicum) has been considered a very important and meaningful industrial microorganism for the production of amino acids worldwide. To produce amino acids, cells require nicotinamide adenine dinucleotide phosphate (NADPH), which is a biological reducing agent. The pentose phosphate pathway (PPP) can supply NADPH in cells via the 6-phosphogluconate dehydrogenase (6PGD) enzyme, which is an oxidoreductase that converts 6-phosphogluconate (6PG) to ribulose 5-phosphate (Ru5P), to produce NADPH. In this study, we identified the crystal structure of 6PGD_apo and 6PGD_NADP from C. glutamicum ATCC 13032 (Cg6PGD) and reported our biological research based on this structure. We identified the substrate binding site and co-factor binding site of Cg6PGD, which are crucial for understanding this enzyme. Based on the findings of our research, Cg6PGD is expected to be used as a NADPH resource in the food industry and as a drug target in the pharmaceutical industry.
|
|
|
KEYWORD
|
|
|
6-Phosphogluconate dehydrogenase, Corynebacterium glutamicum, crystal structure, 6-phosphogluconate, nicotinamide adenine dinucleotide phosphate
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|